FLOW CITOMETRY


The Genetics laboratory at Via degli Apuli site  has been developing  since 1991 flow-cytometric techniques
for studies  of the cell cycle, apoptosis and protein expression in mammalian, drosophila and yeast cells.

The service is equipped with a Beckman Coulter EPICS XL Flow Cytometer


facs

The XL system redefines the standards of flow-cytometric analysis. It combines the analytical power of a research flow cytometer into a compact clinical analyzer ergonomically designed to expedite sample throughput.
The XL system can analyze up to 4 colors of fluorescence from a single air cooled laser. Multi-color applications include a broad range of cell biology/functional studies, e.g.:
   - multiparametric DNA analysis to analyse cell cycle phases, DNA damage and repair, apoptosis, necrosis and mutations in pathways thereof, detection of cell surface markers in cell lineage studies,
The flexibility to change filter elements increases the versatility  of research settings. The XL offers state-of-the-art Digital Signal Processing (DSP) for reliable linearity and drift-free amplification and compensation. The single laser design eliminates concerns regarding multi-beam stability, signal delay and alignment. Compensation is achieved by a unique digital compensation matrix.






CONTACTS:

Dr. Mario Fiore   (responsible for the flow-cytometry utility)

Dr. Enrico Cundari


Tel. +39 06 4457 527
E-mail: mario.fiore@uniroma1.it



USEFUL  SITES:

GIC

Flow Cytometry - Research & Discovery

Instrumentation Laboratory- Italy

Flow Cytometry – Software


       
 
         ACTIVITIES AND EVENTS:


         XXXI CONFERENZA NAZIONALE
         DI CITOMETRIA

         Lucca, 8-11 OTTOBRE 2013
         http://biotec.casaccia.enea.it/GIC/







IMAGES:

  APO                                CC


FLOW CYTOMETRY FACILITY PUBLICATIONS
  • Fiore M, Degrassi F. (1999) Dimethyl sulfoxide restores contact inhibition-induced growth arrest and inhibits cell density-dependent apoptosis in hamster cells. Exp. Cell Res. 25: 102-110
  • M Casenghi, R Mangiacasale, M Tuynder, P Caillet-Fauquet, A Elhajouji, P Lavia, S Mousset, M Kirsch-Volders and E Cundari (1999) p53-independent apoptosis and p53-dependent block of DNA rereplication following mitotic spindle inhibition in human cells. Exp. Cell Res. 250: 339-350.
  • Mangiacasale R., Tritarelli A., Sciamanna I., Cannone M., Lavia P. Barberis M., Lorenzini R. and Cundari E. (2001). Normal and cancer-prone human cells  respond differently to extremely low frequency electromagnetic fields. FEBS Letters, 487: 397-403
  • Fiore M, Zanier R, Degrassi F. (2002) Reversible G(1) arrest by dimethyl sulfoxide as a new method to synchronize Chinese hamster cells. Mutagenesis.17:419-24.
  • Somma, M. P., Fasulo, B., Cenci, G., Cundari, E., and Gatti M. (2002). Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells. Mol. Biol. Cell, 13:2448-2460
  • Foglietti C, Filocamo G, Cundari E, De Rinaldis E, Lahm A, Cortese R, Steinkuhler C. (2006) Dissecting the biological functions of drosophila histone deacetylases by RNA interference and transcriptional profiling. J. Biol. Chem. 281(26): 17968-76.
  • Mattiuzzo M, Fiore M, Ricordy R, Degrassi F. (2006) Aneuploidy-inducing capacity of two widely used pesticides. Carcinogenesis. 27: 2511-8.
  • Vernarecci S, Ornaghi P, Bâgu A, Cundari E, Ballario P, Filetici P. (2008) Gcn5p plays an important role in centromere kinetochore function in budding yeast. Mol Cell Biol. 28:988-996.
  • Ferretti C, Totta P, Fiore M, Mattiuzzo M, Schillaci T, Ricordy R, Di Leonardo A, Degrassi F. (2010) Expression of the kinetochore protein Hec1 during the cell cycle in normal and cancer cells and its regulation by the pRb pathway. Cell Cycle. 9:4174-82.
  • Romagnoli G, Cundari E, Negri R, Crescenzi M, Farina L, Giuliani A, Bianchi MM. (2011) Synchronous protein cycling in batch cultures of the yeast Saccharomyces cerevisiae at log growth phase. Exp Cell Res. 317:2958-68.